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zeaxanthin et al.

Harry Yamamoto yamamoto at UHUNIX.UHCC.HAWAII.EDU
Tue Dec 20 17:54:06 EST 1994


The reference is:

Gilmore AM and Yamamoto HY (1991) J. Chromatogr 543: 137-145.

Thanks for the complement.  I'm glad that people are finding the method 
useful.

Harry Y. Yamamoto


On Tue, 20 Dec 1994, Darren Goetze wrote:

> actually xanthophyll cycle pigments can also be separated on plates as 
> well (see photosynthetica, v24,no3,p412 (1990)). however, in my 
> experience you are best off with a system that can achieve a real 
> separation of lutein and zeaxanthin (no mean feat; they are positional 
> isomers!). i have adapted the excellent protocol of gilmore and yamamoto 
> (j chromatography 1991, but i don't have the ref handy) and had superb 
> results, virtual baseline separation.
> hope this helps,
> best regards,
> darren goetze
> =========
> dept botany
> univ british columbia
> vancouver bc
> canada
> 
> On 19 Dec 1994, albino maggio wrote:
> 
> > 
> > I am looking for methods to measure levels of
> > zeaxanthin, violaxanthin and b-carotene.
> > Is HPLC the only way to separate them?
> > What about quantification?
> > Any reference or suggestion is welcome.
> > Thanks in advance.
> > Albino Maggio
> > 
> > 
> 
> 



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