actually xanthophyll cycle pigments can also be separated on plates as
well (see photosynthetica, v24,no3,p412 (1990)). however, in my
experience you are best off with a system that can achieve a real
separation of lutein and zeaxanthin (no mean feat; they are positional
isomers!). i have adapted the excellent protocol of gilmore and yamamoto
(j chromatography 1991, but i don't have the ref handy) and had superb
results, virtual baseline separation.
hope this helps,
best regards,
darren goetze
=========
dept botany
univ british columbia
vancouver bc
canada
On 19 Dec 1994, albino maggio wrote:
>> I am looking for methods to measure levels of
> zeaxanthin, violaxanthin and b-carotene.
> Is HPLC the only way to separate them?
> What about quantification?
> Any reference or suggestion is welcome.
> Thanks in advance.
> Albino Maggio
>>
