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Culture of barley mesophyll protoplasts

STEVEN FOOTITT steven at essex.ac.uk
Tue Apr 11 14:47:42 EST 1995

I am trying to culture barley mesophyll protoplasts for transient gene 
expression studies of Calvin cycle genes.
Unfortunately the viability in culture over 12-20 hours is very variable.
I have tried various culture solutions e.g. Sarhan and Cesar, Sheen, and 
Lazzeri. Would a layer of nurse cells (Brome) help?
Any suggestions would be appreciated.

Dr. Steven Footitt
Dept. of Biology
University of Essex
United Kingdom

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