IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

chlorophyll extraction from mosses

John Berges berges at BNLUX1.BNL.GOV
Tue May 2 07:13:59 EST 1995

Re: different extraction protocols for chl a

In microalgae, 90% acetone is the most common solvent, but certain species
such as the chlorophytes and cyanobacteria are problematic.  There are several
options, each of which works better under some circumstances: 100% methanol,
acetone with DMSO, dimethylformamide, etc.  How you prepare the extract
i.e. grinding in solvent with a tissue homogenizer, extracting liquid N2
freeze/thawed cells, or sonication ia also an important factor.
You should also be aware of possible degradation of chla, which can occur
quite rapidly in some species.

More information about the Photosyn mailing list

Send comments to us at archive@iubioarchive.bio.net