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Photosynthesis and Chloroplast Experiment Assistance

CHAigle at aol.com CHAigle at aol.com
Sat Nov 30 14:58:37 EST 1996

I am a 15-year-old high school student and am working on a science project on
photosynthesis.  I am trying to use a variety of plants to see which one's
chloroplasts will survive and produce oxygen outside of their hosts.

I found "photosyn at net.bio.net" while browsing the Intermet and thought that
one of your members might be be able give me valuable advice.

I have, I believe, successfully separated chloroplasts from spinach leaves
using a modified Whatley and Arnon procedure at home.  For example, my bell
jar is a cheese board cover of about 1400 cm3 volume.  I produced
chloroplasts from 25 g spinach leaves with a .25 mol saline solution made
from halite.

My basic problem is determining whether oxygen has been produced.  I have
tried using a glowing match to see if it flared.  I couldn't be sure if I was
successful.  Do you have any suggestions to help me determine, at home,
whether I have successfully produced oxygen?  Is there, for example, an easy
chemical test I could use?  Are there any special things I should do, or not
do, to help ensure success?  Any advice or suggestions for sources on the
Internet you might have would be greatly appreciated.  I have access only to
a medical centrifuge and microscope (100x) where my mother works.  My school
has a spectrometer I could use.

I thank you in advance for any assistance you might be able to give me.


Corinne Milliken
(I am using my dad's e-mail account)

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