You need to ensure that the thylakoids are very well homogeneised before
the dark incubation step. If you have small clumps of thylakoid material,
then you will get PSI contamination in the final PSII membrane fragment
samples.
Paul Smith
ANU
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c/- Department of Chemistry
Australian National University
ACT 0200
AUSTRALIA
Ph: 61 2 6249 3074
Fax: 61 2 6249 0760
email: Paul.Smith at ANU.edu.au
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