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Maize chloroplast isolation

Nobody nobody at hgmp.mrc.ac.uk
Mon Nov 26 04:48:17 EST 2001

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<DIV><FONT face=3DArial size=3D2>Dear Photosynthesis Group:</FONT></DIV>
<DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>Im a Master Student from Portugal. Im doing=
research about the effects of some triazine herbicides in the z-scheme of th=
photosynthetic process. The plant is maize or corn (Zea mays L.). I try to
measure the chloroplastic activities with the ACMA probe in this plant.
Sometimes I have a good quenching, sometimes not. Can you help me to find a
suitable isolation (grinding) and buffer mediums to this C4 plant? What are =
differences in the isolation process of maize mesophyll chloroplasts and the
bundle sheath chloroplasts of this "instable" plant? Thank you all in
<DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>All my best regards from Portugal.</FONT></=
<DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>Victor Galhano</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>University of Tr=B7s-os-Montes e Alto
<DIV><FONT face=3DArial size=3D2>Vila Real-Portugal</FONT></DIV></BODY></HTM=


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