Joan Hudson asked:
>Could someone supply me with the recipe or the reference for the
>recipe for Hoyer's mountant/fixative. Thank you.
Here it is:
Hoyer's Medium
gum arabic 30.0 g
glycerol 16.0 ml
chloral hydrate 200.0 g
distilled water 50.0 ml
Dissolve gum in water, a little heat helps; add chloral hydrate, then glycerol.
This recipe from Humason, Gretchen L. 1967. Animal Tissue Techniques.
2nd Ed. San Francisco: W. H. Freeman. pages 131-132. With this recipe
Humason cites Beek, R. M. 1951. Improvements in the squash technic for
plant chromosomes. El Aliso. 3: 131-133.
See also
Anderson, L. E. 1954. Hoyer's solution as a rapid permanent mounting
medium for bryophytes. Bryologist 57: 242-244.
[CAUTION: I don't have the info at my finger tips but I think chloral
hydrate is carcinogenic. You will want to check before using this with
students or even for yourself.]
Dr. David W. Kramer
Department of Plant Biology
Ohio State University at Mansfield
1680 University Drive
Mansfield, OH 44906
(419) 755-4344 FAX: (419) 755-4367
e-mail: kramer.8 at osu.edu