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blue-green pigment in spinach extract

Michele Bridgers BRIDGERS at NAT.PEMBROKE.EDU
Mon Oct 30 08:14:19 EST 1995


To:            plant-ed at net.bio.net
From:          SCHMID at butler.edu
Subject:       blue-green pigment in spinach extract
Date:          27 Oct 1995 09:44:45 -0700

Does anyone remember the identity of the blue-green pigment
that is extracted from spinach with acetone and runs down with
or below the xanthophylls when you separate pigments by TLC?

(No, not chlorophyll....)

Kathy Schmid
Dept. of Biological Sciences
Butler University
Indianapolis, IN  46208
schmid at butler.edu  

When we do this experiment, we get 4 bands: a blue green that is 
Chlorophyll a, an olive green that is chlorophyll b, a pale yellow 
that is xanthophyll, and an orange-yellow that is carotene.  (These 
are separated with 95 parts ether and 5 parts acetone, but extracted 
with acetone.)

S. Bridgers, Pembroke State Univ.



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