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mysterious pigment

Larry D Nooden ldnum at BIOLOGY.LSA.UMICH.EDU
Mon Oct 30 08:42:45 EST 1995


My vote is for phaeophytin.  Basically what happens is that acids 
released from the vacuoles during homogenization of the cells cause the 
Mg+2 in chlorophyll to be replaced with H+, so you will need to use a 
strong buffer ca. pH 7.  See Canfield et al. in Annals of Botany 75: 143- 
(1995) for discussion and references.

Larry D. Nooden                           Telephone 313-764-4436
Biology Dept., University of Michigan     FAX       313-747-0884
Ann Arbor, MI 48109-1048
            URL: http://www.biology.lsa.umich.edu/~ldnum/
 








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