>At 9:45 AM 11/27/96 -0800, Carl Johnson wrote:
>>Could someone please explain the difference between a "dihaploid" (e.g.,
>>Nicotiana plumbaginifolia) and a diploid?
>> Thanks,
>> Carl Johnson
Ross Koning wrote:
>A guess: a chromosome-doubled haploid?
>>This topic comes up with regard to tissue-culture
>derived plants coming from microspores that double
>spontaneously to make completely-homozygous "diploids".
>
That is right. However,the spontaneous doubling of chromosomes is very
infrequent. So, dihaploids are generally induced from haploid plants
(derived from microspores by in vitro anther culturing) by a brief
colchicine (a plant alkaloid) treatment. Cochicine allows chromosomal
doubling but stops the cell duplication.
Subbaiah Chalivendra
Subbaiah Chalivendra
S-27 Turner Hall
Dept Agronomy, 1102 S. Goodwin Ave (phone: 333-9743)
2034 S. Orchard st, #C (phone 367-8834)
