>At 2:52 PM 10/7/96 -0700, Michael Joseph Kerkman wrote:
>>Ok! I am sorry for the question about the agar. I should have been more
>>specific. Thank you to those of you who helped me out seriously. What I
>>think happened is that we only had about a .8% agar concentrate, it may
>>have been a typo in the recipe, or we could have just read it wrong and
>>messed up.
>>Good! I think you've got it figured out. 8 grams per liter
>of agar will probably be VERY soft agar. I like to use 12 or
>15 grams per liter, and sometimes use 20 grams per liter
>depending on how "stiff" I want the gel to be. Good Luck on
>the re-make!
>
Actually, .7% agar is pretty common in plant tissue culture and .8% is
pretty standard for bacterial applications. I just poured a batch of .7%
and it gelled really nice. When I've had this problem in the past it is
usually because I didn't stir and bring it to a full boil BEFORE
autoclaving it. I know, the autoclave boils it, but it just doesn't do the
job alone. Any explanations for that one??
Best wishes!
Chris
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