I am a 4th year biology student at Wilfrid Laurier. I am looking for
some information on how to measure carotoid concentrations from leaf
discs. I have a method where leaf discs are placed in a test tube and
incubated in DMSO for 1 hour at 60 degrees celcius. The absorbance of a
specific volume of the sample is then taken at 450 nm. The problem I
have now is there being no formula to plug this value into. This method
is also used for chlorophyll a and b but I have formulas to derive the
concentration of chlorophyll.
I would appreciate any help,
Thanks, Laurie.
