Plant-eders,
I'm teaching Molecular Biology in the fall and we've been using an
Arabidopsis cDNA library for a semester-long project in which students
isolate plasmid DNA from random colonies, construct maps, sequence the
ends, then attempt to identify them using BLAST.
[http://ip25-204.pc.jmu.edu/DOCS/mbio/mblab/mbpage1.html] The project
works well but, because the Arabidopsis genome is being sequenced, the odds
of finding something novel are getting worse and worse. What we would like
is a new cDNA library preferable from an odd organism (not well studied at
the molecular level and not necessarily a plant). We would also prefer
that it be in ZipLox vector because plasmids are very easy to rescue. If
you or a colleague have such a library and would be willing to share,
please contact me (monroejd at jmu.edu).
Thanks!
Jon
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Jonathan Monroe
Associate Professor
Department of Biology
James Madison University
Harrisonburg, VA 22807
voice: 540-568-6649 (office)
540-568-6045 (lab)
fax: 540-568-3333
e-mail: monroejd at jmu.edu
www.jmu.edu/biology/biofac/jmonroe/jmonroe.html
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