Here are recipes for extraction and chromatography of anthocyanins:
Grind plant tissue in a mixture of 18% (v/v) n-propanol, 1% HCl, and 81%
water. This is a fairly standard procedure, it will extract anthocyanins but
not chlorophylls and carotenoids. For chromatography: make a mixture of 40%
n-butanol, 50% distilled water, and 10% glacial acetic acid. Shake well, let
the two layers separate, separate them with a separatory funnel. Use the
upper layer from chromatography on paper or cellulose thin layer.
Neal M. BARNETT, Associate Professor
Department of Cell Biology & Molecular Genetics
2208 H.J. Patterson Hall
University of Maryland College Park MD 20742-5815
Email: nb2 at umail.umd.edu Phone: 301-405-1638
