Dear plant eders,
Student in my lab is using calcoflour white M2R (Sigma) to look for
regeneration of cell walls in wheat protoplasts. The problem she is having
is that calcoflour precipitates in the osmoticum solution which consist of:
500 mM sorbitol, 5 mM MES, 1 mM CaCl2, pH 6.0. Does anybody in the list
have a suggestion to overcome this problem?
Thanks
Magaly
Magaly Rincon-Zachary
Dept. of Biology
Midwestern State University
3410 Taft Blvd.
Wichita Falls, TX 76308
Ph: (940) 397-4254
Fax: (940) 397-4831
e-mail frinconm at nexus.mwsu.edu
