At 12:47 PM 1/18/99 -0800, you wrote:
>In an effort to persuade students (and faculty) that plant physiology has
>something to offer students interested in biotechnology, I am trying to add
>some experiments
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Virginia,
Get a copy of:
Plant Biotechnology : A Laboratory Manual by Robert J. Lebowitz
Amazon Price: $37.55 Paperback Spiral edition (January 1995)
WCB/McGraw-Hill; ISBN: 0697151190 Availability: This title usually ships
within 4-6 weeks.
His lab manual does what you are talking about.
I tried the boxes but contamination was overwhelming. Kits
are available from some companies for laminar benches.
You might want a video, try the URL:
http://www.zeta.org.au/~brianc/pcc.htm
It is a good video and will help with asepsis but is in a
non-USA format. The distributor in Australia are almost
incompetent. (sorry about the language)
If you do a complete Internet search for a search string
"laminar and airflow and bench and kit" I bet you will
turn up something. I was in your position three years ago
and finally got a donation of biological cabinets and
purchase two huge laminars from military surplus through
the State Agency for Surplus Property that are also on
the Internet at:
http://www.drms.dla.mil/index.html
I also found student that had taken Microbiology were
self confident to extreme and unable to adjust to the
more strict asepsis required of plant media. I have to
almost reprogram them to avoid contamination.
Let me know if I can help by email.
