Magaly Rincon-Zachary wrote:
>Does anybody know of an easy staining procedure to visualize sieve tube
>members in fresh tissue?
>>Thank you very much.
>>Magaly
There is a stain that stains callose a bright blue. From memory, which may be wrong, the recipe is 0.1% (or was it 0.01% ?) aniline blue in a bicarbonate buffer at pH 8. The aniline blue turns to brown over a couple of days, so the solution should be made up shortly before use from a concentrated solution of aniline blue in ethanol.
Cheers
Bob Vickery
vickery at mpx.com.au
