I have also had bad luck with Carolina's inoculum. You can dig up a clover
outside, remove and surface-sterilize some nodules, crush them in sterile
0.2 M sucrose (or water), and streak on yeast-mannitol agar. In less
than a
week, you will have your own inoculum. Then you can do experiments like
comparing +/- N fertilizer on nodule growth.
Nancy Artus
West Chester University
> ----------
> From: pelkki at svsu.edu> Sent: Thursday, October 4, 2001 5:16 PM
> To: plant-ed at net.bio.net> Subject: Root nodules
>> Hi, all!
>> I've been trying to grow clover, soybean, etc. to show root nodules to
> students without success. Can anyone recommend a plant that does well
> and what is the amount of time needed to grow these plants from seeds in
> order to see root nodules? I've been using the rhizobium innoculum from
> Carolina Biological... it's a black dust.
>> Thanks!
> Kathy Pelkki
>pelkki at svsu.edu>> ---
>>
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