The American Biology Teacher Volume 51, March 1989, pp. 165-167
describes a protocol for establishment and maintenance of carrot callus
and initiation of somatic embryogenesis from callus. Each stage of the
process uses Murashige and Skoog with Minimal Organics (MSMO) medium
with different levels of 2,4-D added. Callus initiation requires 1 mg /
L of 2,4-D, callus maintenance requires 0.4 mg / L and somatic
embryogenesis is initiated when callus is placed on media lacking 2,4-D.
Enjoy!
At 01:33 AM 2/14/2003 +0000, you wrote:
> I am looking into having my plant physiology students culture carrot
> tissue. Sigma lists a number of different pre-made media from which
> to chose. Does anyone have suggestions regarding which medium might
> be best for carrot callus growth and which would be the choice for
> carrot shoot regeneration from callus?
>> Bob
> ---
Mark D. Spiro
Department of Biology
Bucknell University
Lewisburg, PA 17837
spiro at bucknell.edu
phone: (570) 577-3486
fax: (570) 577-3537
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