KCN effect on DCPIP reduction of chloroplast

[David Alan Walker]

Just a suggestion. Chloroplast prep will, very likely, have 
residualcatalase activity.  Seem to remember that reduced DCPIP may, in 
somecircumstances, be subject to catalactic reoxidation. Addition of KCN 
would then accelerate net reduction by inhibiting reoxidation. Could be 
tested by adding a little commercial catalase +/- KCN.


  From David Walker University of
Sheffield, UK.
  http://www.oxygraphics.co.uk/