Thanks,
So in effect, it can be grown in lab. Could you point me to resources
where the optimum growth parameters are mentioned or something like
that?
I'm an masters in technology student in a college in India and am
looking to do a project in a bioprocess control system design to
culture this bacteria.
Thanks,
Mohit.
Jon Monroe wrote:
> Hi Mohit,
>> I used to work on legume root nodules so I'll try to answer your
> question. Rhizobia have two major lifestyles, free living in soil (or a
> culture flask), and as an intracellular symbiont in root nodules of
> legumes. In the free living state they have cell walls and are easy to
> grow. During root nodule development Rhizobia differentiate in various
> ways - they become larger and sometimes branched, express a number of
> different genes associated with N2 fixation, and apparently lose the
> ability to dedifferentiate and reproduce! (I didn't know that when I
> started this response). Some strains do grow slowly (e.g.
> Bradyrhizobium, also referred to as the "slow growers"), but they do
> grow in culture.
>> Hope this helps.
>> Jon
>>>schouse at gmail.com wrote:
> > Hi,
> >
> > Is it possible to culture rhizobium bacteria in a lab bioreactor? I've
> > read somewhere that these bacteria lack a cell wall and cannot be
> > easily cultured?
> >
> > Regards,
> > Mohit.
> >
> > _______________________________________________
> > Plant-ed mailing list
> > Plant-ed at net.bio.net> > http://www.bio.net/biomail/listinfo/plant-ed>>> --
> ---------------------------------
> Jonathan Monroe, Professor
> Department of Biology, MSC 7801
> James Madison University
> Harrisonburg, VA 22801
> office: 304 Burruss Hall
> phone: 540-568-6649
> fax: 540-568-3333
> e-mail: monroejd at jmu.edu>http://www.jmu.edu/biology/> ---------------------------------
