In article <2lthr1$9qj at nnrp.ucs.ubc.ca>, marcy at unixg.ubc.ca (Marcy Lynn
Pettypiece) wrote:
>>> If one were to Deciliate a unicellular organism, such as Chlamydomonas,
> would the cilia gorw back quickly or not? and does the tubulin come from
> pools, protein synthesis or both? and to what degree? perhaps one could
> inhibit protein synthesis and see hoe big the tubulin reserves are.
> Any comments or suggestions?
With Chlamydomonas, what happens is that the flagella grow back over the
course of 1-2 hours. In the presence of cycloheximide, some regeneration
occurs but the flagella reach only about half of normal length, suggesting
that the tubulin pool isn't big enough to make two full-length flagella.
The classic paper is Rosenbaum et al. (1969). Flagellar elongation and
shortening in Chlamydomonas. The use of cycloheximide and colchicine to
study the synthesis and assembly of flagellar proteins. J. Cell Biol. 41,
600-619.
Elizabeth Harris
chlamy at acpub.duke.edu
