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From: Self <ZOOL/GOODE>
To: AUN103 at URIACC.URI.EDU (Linda Hufnagel),
Subject: Re: Antibodies against cytoskeletal proteins
Date: Fri, 6 May 1994 16:16:41
Linda:
The best broad spectrum anti-tubulin that we've used is the rat mc-
antitubulin of John Kilmartin (JCB 93:576); it works on the ciliates
we've tried. Also, Andre Adoutte made a rabbit polyclonal against
paramecium tubulin that works well on ciliates, of course, and I
have a small amount of it.
You may also want to try our backscatter EM method for examining MTs
under the surface of cells (JEM Tech 5:263).
Dennis
