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Antibodies against cytoskeletal proteins

Dr. Dennis Goode GOODE at ZOOL.UMD.EDU
Fri May 13 10:37:43 EST 1994

------- Forwarded Message Follows -------

From:     Self <ZOOL/GOODE>
To:       AUN103 at URIACC.URI.EDU (Linda Hufnagel),
Subject:  Re: Antibodies against cytoskeletal proteins
Date:     Fri, 6 May 1994 16:16:41

The best broad spectrum anti-tubulin that we've used is the rat mc-
antitubulin of John Kilmartin (JCB 93:576); it works on the ciliates 
we've tried. Also, Andre Adoutte made a rabbit polyclonal against 
paramecium tubulin that works well on ciliates, of course, and I 
have a small amount of it.
You may also want to try our backscatter EM method for examining MTs 
under the surface of cells (JEM Tech 5:263).

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