Veljo Kisand <veljo.kisand at limno.uu.se> wrote:
>Hi netters!
>>Does anybody have or know how I can get minicells which I like to stain
>with some fluorescence marker and use for protozoa grazing experiments on
>these bacterial mini cells?
>
Minicells-you mean small DNA-less cells, right? I know that for E. coli it is very easy to purify minicells. You grow the mutant strain (minB) which produces minicells periodically and then put the cells on a sucrose gradient to separate normal size cells from minicells. I don't have a reference-but it was used as a way of preferentially labelling plasmid encoded proteins (plasmids go into minicells) by F. Neidhardt as well as others.
Anne