IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

minicells

Anne Farewell gmmaf at gmm.gu.se
Mon Aug 7 13:41:35 EST 1995


Veljo Kisand <veljo.kisand at limno.uu.se> wrote:
>Hi netters!
>
>Does anybody have or know how I can get minicells which I like to stain
>with some fluorescence marker and use for protozoa grazing experiments on
>these bacterial mini cells?
>

Minicells-you mean small DNA-less cells, right?  I know that for E. coli it is very easy to purify minicells.  You grow the mutant strain (minB) which produces minicells periodically and then put the cells on a sucrose gradient to separate normal size cells from minicells.  I don't have a reference-but it was used as a way of preferentially labelling plasmid encoded proteins (plasmids go into minicells) by F. Neidhardt as well as others.
Anne




More information about the Protista mailing list

Send comments to us at archive@iubioarchive.bio.net