Hi,
I'm a PhD student working at the Centre for Limnology (the Netherlands). I'm
working on the molecular identification of aquatic protozoa. Using several sequence
databanks I constructed a universal eukaryotic 18S rDNA PCR primer set. The PCR
product is small enough to separate on a DGGE gel. The method seems to be very
promising in screening large numbers of samples on eukaryotic biodiversity. Some
groups of Protist have up to 4 mismatches in the forward primer and I need to test
the PCR on these sequences. There is only one problem. I don't have these species
in culture so that's why i'm writing this letter. I would like to have DNA of the
next species or groups:
-Bodo caudatus (or any other Kinetoplastida).
-Hexamita sp. (or any other Diplomonadida).
-Tetrahymena sp. (or any other Oligohymenophorea)
-Plasmodium sp. (or any other Haemosporina)
-Euglena gracilis.
If somebody out there has cultures or DNA isolations of one of these species I
would really like some of it....if they wish to share it.
PLease contact me!
Erik van Hannen
Centre for Limnolgy voice: +31 294 239315
Rijksstraatweg 6 fax : +31 294 232224
3631 AC Nieuwersluis email: vanhannen at cl.nioo.knaw.nl
The Neterlands
