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Nathaniel A Hawkins hawkina at mail.auburn.edu
Wed Sep 11 09:32:10 EST 1996

I am an undergraduate student who has an interest in trichomonad 
flagellates.  I would like to stain the nucleus with a fluorescent dye 
but have had no luck.  DAPI and propidium iodide give no reaction 
although the positive control (Tetrahymena) stains beautifully.  I would 
appreciate any information on procedures for fluorescently labelling 
trichomonad nuclei for observation with microscopy, especially confocal 
microscopy.  Also, does anyone know why the "augusta" group can be 
cultured in vitro and the "muris" group can not?  Thank you for your help.

Nathaniel Hawkins
hawkina at mail.auburn.edu

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