Dear Nathaniel,
we have no experience in labelling TRICHOMONAD nuclei, but for similar
purposes we use Hoechst Dye (Bisbenzimid), purchased from Ridel-de-Haen
in Hannover, FRG. As vital stain it is applied as 10mikroM solution for
several hours, on fixed cells we use 100mikroM for 10 min. and rinse
twice.
I can't promise, that this works with your cells, it's just a suggestion.
Ralf Breuker, at the
Department of Cell Morphology
NDEF 05 / Ruhr University Bochum
44780 Bochum, FRG
