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Two post-doctoral position

Vaulot Daniel vaulot at sb-roscoff.fr
Tue Dec 14 05:47:58 EST 1999


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=20
Two post-doctoral positions available (Dec 99)
Molecular Ecology of Marine Picoplankton

      Post doctoral position - Prokaryotic picoplankton : University of =
Warwick, UK=20
      Post doctoral position  - Eukaryotic picoplankton : Station =
Biologique de Roscoff, France=20

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Postdoctoral Research Assistant=20
in Molecular Ecology=20
focusing on=20

A genetic analysis of the spatial and temporal dynamics=20
of photosynthetic picoplankton communities

Dept. of Biological Sciences, University of Warwick, Gibbet=20
Hill Road, Coventry, CV4 7AL, UK

This EU-funded position is available starting April 2000 to investigate =
how genetic diversity among marine photosynthetic prokaryotes of the =
genera Synechococcus and Prochlorococcus is correlated with =
environmental adaptation. The project is part of an EU consortium =
project PICODIV (www.sb-roscoff.fr/Phyto/PICODIV/index.html) with =
participating laboratories in France (co-ordinator), Germany, Spain & =
Norway. You should have a PhD and experience in molecular biology. You =
will be highly motivated and have a particular interest in applying =
molecular and biochemical techniques to the biological oceanography of =
ecologically important microorganisms.=20
The post is for three years at a salary on the RA1A scale.

Informal enquiries to Dr Dave Scanlan=20
tel : +44-24 76-528363; fax :44-24 76-523701 e-mail : =
dp at dna.bio.warwick.ac.uk.=20
Information on applying can be obtained at : =
http://www.csv.warwick.ac.uk/jobs/=20

Please supply full C.V., a summary of research interests/experience, and =
details of two referees.

Additional details for this post:
PICODIV : Genetic analysis of the spatial and temporal dynamics of =
photosynthetic picoplankton communities. Molecular biology techniques =
will be used to investigate how genetic diversity among marine =
photosynthetic prokaryotes is correlated with particular environmental =
adaptation. We have recently observed genotypic variation among natural =
populations of the marine cyanobacterium Prochlorococcus, which =
dominates the picophytoplankton in the world's oceans, associated with =
different depths in a stratified water column using 16S rRNA analysis =
and dot blot hybridisation technology (West & Scanlan 1999). Suitable =
oligonucleotide probes will be developed to extend this technology to =
the marine Synechococcus group. The spatial and temporal distribution of =
the major Prochlorococcus and Synechococcus genotypes will then be =
determined in natural water columns during a calendar year from the =
English Channel, Mediterranean Sea, North Sea and Gulf of Aqaba [Red =
Sea] Eilat, Israel, using various molecular ecological techniques =
including FISH. This will be compared with data obtained using =
denaturing gradient gel electrophoresis (DGGE), to investigate total =
picophytoplankton community genetic diversity. Genotypic variation will =
be correlated with physical and chemical properties of the water column =
and to the phenotypic response of Prochlorococcus and Synechococcus =
populations to environmental variables (see e.g. Scanlan et al., 1997).=20
References
West, N.J., and D. J. Scanlan. (1999). Niche-partitioning of =
Prochlorococcus populations in a stratified water column in the eastern =
North Atlantic Ocean. Appl. Environ. Microbiol. 65: 2585-2591
Urbach, E., Scanlan, D.J., Distel, D.L., Waterbury, J.B., and Chisholm =
S.W. (1998). Rapid diversification of marine picophytoplankton with =
dissimilar light harvesting structures inferred from sequences of =
Prochlorococcus and Synechococcus (Cyanobacteria). J. Mol. =
Evol.46:188-201.=20
Scanlan, D.J., Silman,N.J., Donald, K.M., Wilson, W.H., Carr, N.G., =
Joint, I., and Mann N.H. (1997). An immunological approach to detect =
phosphate stress in populations and single cells of photosynthetic =
picoplankton. Appl. Environ. Microbiol. 63: 2411-2420.


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Postdoctoral Position=20

Assessing and monitoring the diversity of eukaryotic photosynthetic =
picoplankton

Station Biologique de Roscoff
CNRS et Universit=E9 Pierre et Marie Curie
BP 74, 29682 Roscoff, France

This position will be funded by the FP5 EU program PICODIV to start in =
April 2000 which focuses on the diversity of eukaryotic photosynthetic =
picoplankton in coastal and oceanic waters.=20

Emphasis will be on the following aspects:

  a.. Isolation and description of novel picoplankton strains from =
coastal and oceanic waters using a combination of techniques (electron =
microscopy, HPLC pigment signature, rDNA gene sequences).
  b.. Direct determination of the environmental diversity from rDNA gene =
sequences
  c.. Quantitative use of rDNA probes (quantitative PCR) to monitor =
picoplankton diversity at three european coastal sites
Skills :

  a.. The candidate is expected to have a strong academic background, to =
have published in international peer-reviewed journals and to be able to =
conduct independent research
  b.. Experience with modern taxonomical approaches inolving rDNA genes =
on bacteria or unicellular eukaryotes (cloning and sequencing of =
environmental samples, phylogenetical analysis, DGGE, rRNA probes, =
quantitative PCR) will be critical
  c.. Experience in algal taxonomy and algal culture will be highly =
desirable
  d.. Working knowledge of the French language will be an essential =
prerequisite for rapid integration in our team
Starting date: Spring 2000
Duration: 24 to 30 months

Additional information on PICODIV : =
www.sb-roscoff.fr/Phyto/PICODIV/index.html
Additional information on the Roscoff group : =
www.sb-roscoff.fr/Phyto/phyto_en.html

If interested please supply by email:
- C.V. with published papers
- Summary of research interests/experience
- Adress of two referees.
Supply by regular mail a copy of each published paper

Send all materials to Daniel Vaulot (vaulot at sb-roscoff.fr)
Oceanic Phytoplankton team
Station Biologique de Roscoff
CNRS et Universit=E9 Pierre et Marie Curie
BP 74, 29682 Roscoff, France
Ph: +33 (2) 98 29 23 34; fax :+33 (2) 98 29 23 24=20


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<DIV>&nbsp;
<P align=3Dcenter><FONT size=3D4><STRONG>Two post-doctoral positions =
available (Dec=20
99)<BR>Molecular Ecology of Marine Picoplankton</STRONG></FONT></P>
<TABLE border=3D0 cellSpacing=3D0>
  <TBODY>
  <TR>
    <TD><STRONG>Post doctoral position</STRONG></TD>
    <TD>- Prokaryotic picoplankton</TD>
    <TD width=3D"56%">: <A =
href=3D"mhtml:mid://00000011/#Warwick">University of=20
      Warwick, UK</A></TD></TR>
  <TR>
    <TD><STRONG>Post doctoral position </STRONG></TD>
    <TD>- Eukaryotic picoplankton</TD>
    <TD width=3D"56%">: <A =
href=3D"mhtml:mid://00000011/#Roscoff">Station=20
      Biologique de Roscoff, France</A></TD></TR></TBODY></TABLE>
<HR color=3D#0000ff>

<P align=3Dcenter><A name=3DWarwick><FONT face=3DArial><B>Postdoctoral =
Research=20
Assistant</B></FONT></A><FONT face=3DArial><B> <BR>in Molecular Ecology=20
<BR>focusing on <BR><BR></B></FONT><FONT color=3D#ff0000 =
face=3DArial><B><I>A=20
genetic analysis of the spatial and temporal dynamics <BR>of =
photosynthetic=20
picoplankton</I></B></FONT><FONT face=3DArial><I> </I></FONT><FONT =
color=3D#ff0000=20
face=3DArial><B><I>communities</I></B></FONT></P>
<P align=3Dcenter><FONT size=3D3><B>Dept. of Biological Sciences, =
University of=20
Warwick, Gibbet <BR>Hill Road, Coventry, CV4 7AL, UK</B></FONT></P>
<P><FONT face=3DArial size=3D2>This EU-funded position is available =
starting April=20
2000 to investigate how genetic diversity among marine photosynthetic=20
prokaryotes of the genera <I>Synechococcus</I> and =
<I>Prochlorococcus</I> is=20
correlated with environmental adaptation. The project is part of an EU=20
consortium project PICODIV (</FONT><A=20
href=3D"http://www.sb-roscoff.fr/Phyto/PICODIV/index.html"><FONT =
face=3DArial=20
size=3D2>www.sb-roscoff.fr/Phyto/PICODIV/index.html</FONT></A><FONT =
face=3DArial=20
size=3D2>) with participating laboratories in France (co-ordinator), =
Germany,=20
Spain &amp; Norway. You should have a PhD and experience in molecular =
biology.=20
You will be highly motivated and have a particular interest in applying=20
molecular and biochemical techniques to the biological oceanography of=20
ecologically important microorganisms. <BR>The post is for three years =
at a=20
salary on the RA1A scale.<BR><BR>Informal enquiries to Dr <STRONG>Dave=20
Scanlan</STRONG> <BR>tel : +44-24 76-528363; fax :44-24 76-523701 e-mail =
:=20
</FONT><A href=3D"mailto:dp at dna.bio.warwick.ac.uk"><FONT face=3DArial=20
size=3D2>dp at dna.bio.warwick.ac.uk</FONT></A><FONT face=3DArial =
size=3D2>.=20
<BR>Information on applying can be obtained at : </FONT><A=20
href=3D"http://www.csv.warwick.ac.uk/jobs/"><FONT face=3DArial=20
size=3D2>http://www.csv.warwick.ac.uk/jobs/</FONT></A><FONT face=3DArial =
size=3D2>=20
<BR><BR>Please supply full C.V., a summary of research =
interests/experience, and=20
details of two referees.<BR><BR><STRONG>Additional details for this=20
post</STRONG><B>:<BR></B><STRONG>PICODIV</STRONG> : Genetic analysis of =
the=20
spatial and temporal dynamics of photosynthetic picoplankton =
communities.=20
Molecular biology techniques will be used to investigate how genetic =
diversity=20
among marine photosynthetic prokaryotes is correlated with particular=20
environmental adaptation. We have recently observed genotypic variation =
among=20
natural populations of the marine cyanobacterium <I>Prochlorococcus</I>, =
which=20
dominates the picophytoplankton in the world's oceans, associated with =
different=20
depths in a stratified water column using 16S rRNA analysis and dot blot =

hybridisation technology (West &amp; Scanlan 1999). Suitable =
oligonucleotide=20
probes will be developed to extend this technology to the marine=20
<I>Synechococcus</I> group. The spatial and temporal distribution of the =
major=20
<I>Prochlorococcus</I> and <I>Synechococcus</I> genotypes will then be=20
determined in natural water columns during a calendar year from the =
English=20
Channel, Mediterranean Sea, North Sea and Gulf of Aqaba [Red Sea] Eilat, =
Israel,=20
using various molecular ecological techniques including FISH. This will =
be=20
compared with data obtained using denaturing gradient gel =
electrophoresis=20
(DGGE), to investigate total picophytoplankton community genetic =
diversity.=20
Genotypic variation will be correlated with physical and chemical =
properties of=20
the water column and to the phenotypic response of =
<I>Prochlorococcus</I> and=20
<I>Synechococcus</I> populations to environmental variables (see e.g. =
Scanlan et=20
al., 1997). <BR><STRONG>References</STRONG><BR>West, N.J., and D. J. =
Scanlan.=20
(1999). Niche-partitioning of <I>Prochlorococcus</I> populations in a =
stratified=20
water column in the eastern North Atlantic Ocean. Appl. Environ. =
Microbiol. 65:=20
2585-2591<BR>Urbach, E., Scanlan, D.J., Distel, D.L., Waterbury, J.B., =
and=20
Chisholm S.W. (1998). Rapid diversification of marine picophytoplankton =
with=20
dissimilar light harvesting structures inferred from sequences of=20
<I>Prochlorococcus</I> and <I>Synechococcus</I> (Cyanobacteria). J. Mol. =

Evol.46:188-201. <BR>Scanlan, D.J., Silman,N.J., Donald, K.M., Wilson, =
W.H.,=20
Carr, N.G., Joint, I., and Mann N.H. (1997). An immunological approach =
to detect=20
phosphate stress in populations and single cells of photosynthetic =
picoplankton.=20
Appl. Environ. Microbiol. 63: 2411-2420.</FONT></P>
<HR color=3D#0000ff>

<P align=3Dcenter><A name=3DRoscoff><FONT face=3DArial><B>Postdoctoral =
Position=20
</B></FONT></A><FONT face=3DArial><B><BR><BR></B></FONT><FONT =
color=3D#ff0000=20
face=3DArial><B><I>Assessing and monitoring the diversity of eukaryotic=20
photosynthetic picoplankton</I></B></FONT></P>
<P align=3Dcenter><B>Station Biologique de Roscoff<BR>CNRS et =
Universit=E9 Pierre et=20
Marie Curie<BR>BP 74, 29682 Roscoff, France</B></P>
<P><FONT face=3DArial size=3D2>This position will be funded by the FP5 =
EU program=20
</FONT><A href=3D"index.html"><FONT face=3DArial =
size=3D2>PICODIV</FONT></A><FONT=20
face=3DArial size=3D2> to start in April 2000 which focuses on the =
diversity of=20
eukaryotic photosynthetic picoplankton in coastal and oceanic waters.=20
</FONT></P>
<P><FONT face=3DArial size=3D2>Emphasis will be on the following =
aspects:</FONT></P>
<UL type=3Ddisc>
  <LI><FONT face=3DArial size=3D2>Isolation and description of novel =
picoplankton=20
  strains from coastal and oceanic waters using a combination of =
techniques=20
  (electron microscopy, HPLC pigment signature, rDNA gene=20
sequences).</FONT></LI>
  <LI><FONT face=3DArial size=3D2>Direct determination of the =
environmental=20
  diversity from rDNA gene sequences</FONT></LI>
  <LI><FONT face=3DArial size=3D2>Quantitative use of rDNA probes =
(quantitative PCR)=20
  to monitor picoplankton diversity at three european coastal=20
sites</FONT></LI></UL>
<P><FONT face=3DArial size=3D2>Skills :</FONT></P>
<UL type=3Ddisc>
  <LI><FONT face=3DArial size=3D2>The candidate is expected to have a =
strong=20
  academic background, to have published in international peer-reviewed =
journals=20
  and to be able to conduct independent research</FONT></LI>
  <LI><FONT face=3DArial size=3D2>Experience with modern taxonomical =
approaches=20
  inolving rDNA genes on bacteria or unicellular eukaryotes (cloning and =

  sequencing of environmental samples, phylogenetical analysis, DGGE, =
rRNA=20
  probes, quantitative PCR) will be critical</FONT></LI>
  <LI><FONT face=3DArial size=3D2>Experience in algal taxonomy and algal =
culture=20
  will be highly desirable</FONT></LI>
  <LI><FONT face=3DArial size=3D2>Working knowledge of the French =
language will be=20
  an essential prerequisite for rapid integration in our =
team</FONT></LI></UL>
<P><FONT face=3DArial size=3D2>Starting date: Spring 2000<BR>Duration: =
24 to 30=20
months<BR><BR>Additional information on PICODIV : </FONT><A=20
href=3D"http://www.sb-roscoff.fr/Phyto/PICODIV/index.html"><FONT =
face=3DArial=20
size=3D2>www.sb-roscoff.fr/Phyto/PICODIV/index.html</FONT></A><FONT =
face=3DArial=20
size=3D2><BR>Additional information on the Roscoff group : </FONT><A=20
href=3D"http://www.sb-roscoff.fr/Phyto/phyto_en.html"><FONT face=3DArial =

size=3D2>www.sb-roscoff.fr/Phyto/phyto_en.html</FONT></A><FONT =
face=3DArial=20
size=3D2><BR><BR>If interested please supply by email:<BR>- C.V. with =
published=20
papers<BR>- Summary of research interests/experience<BR>- Adress of two=20
referees.<BR>Supply by regular mail a copy of each published =
paper</FONT></P>
<P><FONT face=3DArial size=3D2>Send all materials to <STRONG>Daniel =
Vaulot</STRONG>=20
(</FONT><A href=3D"mailto:vaulot at sb-roscoff.fr"><FONT face=3DArial=20
size=3D2>vaulot at sb-roscoff.fr</FONT></A><FONT face=3DArial =
size=3D2>)<BR>Oceanic=20
Phytoplankton team<BR>Station Biologique de Roscoff<BR>CNRS et =
Universit=E9 Pierre=20
et Marie Curie<BR>BP 74, 29682 Roscoff, France<BR>Ph: +33 (2) 98 29 23 =
34; fax=20
:+33 (2) 98 29 23 24 </FONT></P></DIV></BODY></HTML>

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