EU workshop on 'Biodiversity in Aquatic Systems: Environmental Gene
Cloning and Analysis' to be held at the Station Biologique, Roscoff,
Brittany, France between 25-27 June 2000.
Applications are invited to participate in this open workshop, the
major theme of the workshop being the design, screening and analysis
of environmental clone libraries. The format for the workshop will be
a combination of formal talks, small hands-on demonstrations, and
roundtable discussions, the latter concerning the best strategies for
each step in environmental gene cloning & particularly the different
issues that arise when constructing clone libraries.
Accomodation will be available at the Hotel de France, Station
Biologique, Roscoff (210 FF per night including breakfast), Lunch at
the station costs 30 FF & evening dinner can be taken in town. I
include some travel info at the end of the message. A registration
fee of stlg100 is payable so as to fund a small number of overseas
speakers.
Some of the issues we will be discussing are as follows:
Sample collection: volume, filtration method, flow cytometry sorting,
extraction on board ship, preservation
Sample treatment once back in the lab.
Storage time
Choice of gene (only SSU or SSU + other: LSU, rpoC, rbcL etc...)
Use of DNA vs RNA
PCR or not PCR
Cloning strategy (choice of vector)
Clone selection (random, use of probes, RFLP, DGGE, TRFLP, single
base sequencing, SSCP)
Sequencing strategy (automatic sequencer)
Sequence analysis
How is the clone library representative of the initial diversity?
Chimeras : How to detect them? Can one design experiments to test
that?
Effect of rRNA gene numbers (important for eukaryotes such as
dinoflagellates).
The meeting is scheduled for 2.5 days & the basic structure of
the workshop will be :
Sunday Morning Participants welcome; Talks
Afternoon Talks
Practical workshop: set up DGGE
Monday Morning Talks/roundtable
Afternoon Practical workshop: DGGE results
& visualization
Tuesday Morning Roundtable
The workshop precedes a meeting of the EU funded PICODIV grant
see
<http://www.sb-roscoff.fr/Phyto/PICODIV/index.html>http://www.sb-roscoff.fr/
Phyto/PICODIV/index.html and will
include members of this EU consortium as participants in the
workshop: Dr. Daniel Vaulot, Roscoff (PICODIV co-ordinator); Dr Linda
Medlin, Bremerhaven; Dr Dave Scanlan, Warwick; Dr Carlos Pedros-Alio,
Barcelona; & Dr Bente Edvarsen, Oslo as well as a small number of
other outside participants/invited speakers.
I look forward to hearing from you,
Dave Scanlan
-----------------------------------------------------
Dr. D. J. Scanlan,
Royal Society University Research Fellow/Lecturer
Dept. of Biological Sciences,
University of Warwick
Gibbet Hill Road,
Coventry,
CV4 7AL, UK.
Phone +44 24 76 528363 (office) or +44 24 76 522572 (lab)
Fax +44 24 76 523701
Travel Information
Train. Roscoff has a train station which is about 10 min walking from
the Station Biologique. Arriving from Paris you have to change trains
in Morlaix. Total travel time from Paris is about 4-5 hours. Airplane.
The nearest airport is Brest-Guipavas which is about 60 km from
Roscoff. Regular taxi costs about 400F from the airport to Roscoff.
Useful websites for travel information:
SNCF (french railways): http://www.sncf.fr/
Air France: http://www.airfrance.com/
Roscoff web site: http://www.sb-roscoff.fr/
Information about Brittany: http://www.bretagne-online.tm.fr/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. Morgan L. Vis
Environmental & Plant Biology
Ohio University Athens OH 45701 USA
Phone 740 593-1134 Fax 740 593-1130
E-mail vis-chia at ohiou.edu
Internet www.plantbio.ohiou.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Be sure to check out the algae homepage (lots of algae images)
at http://vis-pc.plantbio.ohiou.edu
& the latest Phycological Society of America information
at http://www.psaalgae.org
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