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EU workshop

Dr. Morgan L. Vis vis-chia at oak.cats.ohiou.edu
Thu Mar 16 20:21:35 EST 2000

EU workshop on 'Biodiversity in Aquatic Systems: Environmental Gene 
Cloning and Analysis' to be held at the Station Biologique, Roscoff, 
Brittany, France between 25-27 June 2000.
Applications are invited to participate in this open workshop, the 
major theme of the workshop being the design, screening and analysis 
of environmental clone libraries. The format for the workshop will be 
a combination of formal talks, small hands-on demonstrations, and 
roundtable discussions, the latter concerning the best strategies for 
each step in environmental gene cloning & particularly the different 
issues that arise when constructing clone libraries. 
Accomodation will be available at the Hotel de France, Station 
Biologique, Roscoff (210 FF per night including breakfast), Lunch at 
the station costs 30 FF & evening dinner can be taken in town. I 
include some travel info at the end of the message. A registration 
fee of stlg100 is payable so as to fund a small number of overseas 
Some of the issues we will be discussing are as follows:
Sample collection: volume, filtration method, flow cytometry sorting, 
extraction on board ship, preservation 
Sample treatment once back in the lab. 
Storage time 
Choice of gene (only SSU or SSU + other: LSU, rpoC, rbcL etc...) 
Use of DNA vs RNA 
PCR or not PCR 
Cloning strategy (choice of vector) 
Clone selection (random, use of probes, RFLP, DGGE, TRFLP, single 
base sequencing, SSCP) 
Sequencing strategy (automatic sequencer) 
Sequence analysis 
How is the clone library representative of the initial diversity? 
Chimeras : How to detect them? Can one design experiments to test 
Effect of rRNA gene numbers (important for eukaryotes such as 
The meeting is scheduled for 2.5 days & the basic structure of 
the workshop will be :
Sunday Morning Participants welcome; Talks 
Afternoon Talks 
Practical workshop: set up DGGE 
Monday Morning Talks/roundtable 
Afternoon Practical workshop: DGGE results 
& visualization 
Tuesday Morning Roundtable

The workshop precedes a meeting of the EU funded PICODIV grant 
Phyto/PICODIV/index.html and will 
include members of this EU consortium as participants in the 
workshop: Dr. Daniel Vaulot, Roscoff (PICODIV co-ordinator); Dr Linda 
Medlin, Bremerhaven; Dr Dave Scanlan, Warwick; Dr Carlos Pedros-Alio, 
Barcelona; & Dr Bente Edvarsen, Oslo as well as a small number of 
other outside participants/invited speakers.
I look forward to hearing from you,
Dave Scanlan
Dr. D. J. Scanlan, 
Royal Society University Research Fellow/Lecturer 
Dept. of Biological Sciences, 
University of Warwick 
Gibbet Hill Road, 
CV4 7AL, UK. 
Phone +44 24 76 528363 (office) or +44 24 76 522572 (lab) 
Fax +44 24 76 523701

Travel Information 
Train. Roscoff has a train station which is about 10 min walking from 
the Station Biologique. Arriving from Paris you have to change trains 
in Morlaix. Total travel time from Paris is about 4-5 hours. Airplane. 
The nearest airport is Brest-Guipavas which is about 60 km from 
Roscoff. Regular taxi costs about 400F from the airport to Roscoff.
Useful websites for travel information: 
SNCF (french railways): http://www.sncf.fr/ 
Air France: http://www.airfrance.com/ 
Roscoff web site: http://www.sb-roscoff.fr/ 
Information about Brittany: http://www.bretagne-online.tm.fr/

Dr. Morgan L. Vis 
Environmental & Plant Biology 
Ohio University  Athens OH  45701 USA 
Phone 740 593-1134 Fax 740 593-1130 
E-mail vis-chia at ohiou.edu 
Internet www.plantbio.ohiou.edu 
Be sure to check out the algae homepage (lots of algae images) 
at  http://vis-pc.plantbio.ohiou.edu
& the latest Phycological Society of America information 
at http://www.psaalgae.org

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