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help/advice on amoebal culture

mark schell schell at uga.edu
Mon Nov 24 10:14:44 EST 2003

I am a novice in amoebal culture, trying to grow Acanthamoeba
astronyxis ATCC 30137 in ATCC medium 712 (PYG medium) using 
culture flasks at 30C without shaking.  The rate of growth  seems 
very slow.  Can someone recommend a fast growing 
Acanthamoeba strain
of any species that I may obtain from ATCC to compare its growth 
to the astronyxis I have.  Any other recommendations on liquid or
plate culture would be most appreciated.


Mark A. Schell
Dept. of Microbiology
Univ. of Georgia
Athens, GA 30602
PH:  706-542-2815
FAX  706-542-2674

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