Hi,
Recently I had a problem with some sequences in that it was
giving me a noisy sequence upto 150bp and then good sequence from
150bp to 500bp. But the problem is that eba program clipped the whole
sequence and excluded it from the database. How can I change the eba
parameters so that it becomes more adaptive, i.e. it looks at the
sequence and if it is noisy at the beginning it will clip it and when
it gets to good sequence it will leave it (include it) and then clips
the noisy bit at the end of the sequence instead of clipping
everything as bad sequence.
Also, why does Staden change the Ns in the sequence to - Is there
anyway I can leave the Ns in the sequence, because when I blast the
sequences the BLAST software gives a warning about the -
Thanks.
Rifat.
rifat at icr.ac.uk
