I have been screening the Stratagene lambda Fix II X. laevis genomic
library looking for the promoter region of a gene of interest. We have
screened approximately 4 million clones with several different probes
and are beginning to suspect our gene isn't in this library. Therefore,
I am wondering if anyone out there has or knows of another Xenopus
genomic library other than the one produced by Melton, which I
understand has finally "died".
Also, I would like to get a muscle-specific and constitutive promoter
from Xenopus to use as controls for our preliminary experiments in
making transgenic frogs.
Thanks in advance...
P.S. Thanks to everyone who answered my question regarding mosaicism in