Dear Dr. Malacinski,
As you know I have been working on the transgenic axolotl system for
about a year now as part of my graduate thesis; something it seems you
picked up on from my earlier mailing to the usernet some 6-7 months ago
regarding it and the brief email you sent to me on it.
In regards to Dr. Gordon's recent posting and the results I have thus
obtained with the system being developed here--
In our hands:
1. Electrical activation of the egg is not necessary prior to injection
to obtain cleavage.
2. Direct injection into naturally fertilized eggs yields impressive
results using a reporter plasmid we have had for quite some time but
mosaic expression to varying degrees is still present and thus "true"
transgenesis is not achieved as described by Amaya and Kroll.
3. Injection into the vegetal pole yields quite unsatisfactory results as
compared to injection into the animal hemisphere.
Cheers,
Matthew D. McLean
SUNY Health Science Center
Department of Anatomy/Cell Biology
Syracuse, NY 13210
(315)-464-5120
mcleanm at hscsyr.edu
