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FGF in limb blastema

Saverio Giampaoli giamp at dfb.unipi.it
Mon Apr 6 07:03:36 EST 1998

Is there anyone out there who has the clone of FGF1, FGF2, FGF4 or FGF8
isolated in urodeles? I need the clone of an FGF expressed in limb blastemas
of urodeles (I'm working with Pleurodeles and Triturus) as a good marker for
the proliferative part of the regeneration process. For my investigations I
can use a cDNA (also a part of the coding region is good) that I can
transcribe in RNA for a whole mount, or an antibody for an immuno-staining
(I know that an Ab is very precious...). It is important that the clone (or
the Ab) reacts in urodeles: if the clone comes from an other animal, e.g.
Xenopus or chick, but reacts in urodeles, it will be perfect for me.
I would be very grateful for any help.
Thanks, Save.
Dr.Saverio Giampaoli
Universita' di Pisa
Dipartimento di Fisiologia e Biochimica
Lab. Biologia cellulare e dello sviluppo
via Carducci, 13
I-56010  GHEZZANO (Pisa)

tel.     +39-50-87 83 56
fax      +39-50-87 84 86
e-mail   giamp at dfb.unipi.it

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