Hi all,
I think it is great that this group is up and running but it seems to be
a bit slow. Well instead of complaining I'll do something....
Fact1: Virus "species" are best described as a population of minor
sequence varients. The term quasispecies is usually used here.
Fact2: PCR amplification has an error rate associated with it. The rate
is dependent on the type of polymerase used and other factors such as Mg-2+
concentration and the number of cycles.
Question1: When sequencing a virus, where the clones have been
generated by PCR, how many instances of a particular sequence would have to be
seen before people here would be happy that they have the "correct" sequence and
not a PCR artifact.
Thanks in advance,
Alex
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Alex Jeffries
Prof. R. H. Symons' Lab.
Dept. Plant Science ajeffrie at waite.adelaide.edu.au
Waite Agricultural Research Institute
Glen Osmond, South Australia
Australia
(The University of Adelaide)
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