Dear netters:
PROBLEM
I am trying to separate 1-10 micron Nuclear Polyhedrosis Virus
from microsporidia which is only slightly bigger, ca. 15 microns.
FIRST ATTEMPT
I have tried ultra-centrifugation on a sucrose gradient without
success.
SECOND ATTEMPT
I ran some stock NPV on 0.3% and 0.7% agarose gels for
two hours today. There appeared to be a little movement in the 0.3%
gel. But it could just be that the wells are bulging. I'll know
tomorrow when I continue the run.
Meanwhile, I want to mix up some new gels to run along side the
one's I already have. There was an allusion to electrophoresis on a
sucrose gradient in a 1970's text on plant viruses. I'm tempted to mix
some sugar into the TAE buffer I used to make the 0.3% agarose gel.
Perhaps this will make it viscous enough so that I can further reduce the
concentration of agar?
Any suggestions?
Dan
PS. I'm in Bangkok and don't have direct access to news groups. Please
send replied directly to me at
daniel at chulkn.chula.ac.th
