> Hi Vironetters,
>> I am in need of two protocols. The first one utilises CF-11 (Whatman) to
> isolate ds RNA, the second one uses differential salt precipitations (I
> think 2M and 4M, as well as ethanol). Finally, does anyone have any
> information on the use of glycogen in dsRNA precipitations ?
So - life in virology at net.bio.net!
The best dsRNA extraction protocol - one which works for big plant virus
RNA genomes, as well as 9kb insect picornavirus genomes (Williamson et al.
1988, J gen Virol 69:787-795) - is that of Valverde et al. 1986,
Phytopathology 76:459-465. The refs. describe use of Whatman CF11
cellulose columns/batch procedures for isoln of dsRNA replicative
forms/intermediates from plants and insects.
Good luck! And greetings from the other Down Under.
____________________________________________________________________
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