From: WUGATE::"MAILER-DAEMON at wugate.wustl.edu" "Mail Delivery Subsystem" 14-OCT-1993 10:33:52.55
To: brett at borcim.wustl.edu
CC:
Subj: Returned mail: Host unknown
Received: by WUGATE.DECnet (utk-mail11 v1.6) ; Thu, 14 Oct 93 10:34:56 CDT
Received: by wugate.wustl.edu (5.67a+/WUSTL-0.3)
id AA24268; Thu, 14 Oct 1993 10:17:05 -0500
From: Mail Delivery Subsystem <MAILER-DAEMON at wugate.wustl.edu>
Subject: Returned mail: Host unknown
To: brett at borcim.wustl.edu
----- Transcript of session follows -----
550 uk.ac.crc (TCP)... 550 Host unknown
554 <jclewley at uk.ac.crc>... 550 Host unknown (Authoritative answer from name server)
----- Recipients of this delivery -----
Bounced, cannot deliver:
<jclewley at uk.ac.crc>
----- Unsent message follows -----
Received: by wugate.wustl.edu (5.67a+/WUSTL-0.3) with DECnet
id AA23298; Thu, 14 Oct 1993 10:17:05 -0500
Date: Thu, 14 Oct 1993 10:17:05 -0500
Message-Id: <199310141517.AA23298 at wugate.wustl.edu>
From: brett at borcim.wustl.edu
To: "jclewley at uk.ac.crc"@WUGATE.wustl.edu
Subject: RE: Expression of viral antigens
Well, call this a biased opinion, but here at Wash U we are able to get very
large expression yields in mammalian cells using a Sindbis virus expression
vector. Some references:
London, et al. PNAS V89 pp207-211 (1992)
Hahn, et al. PNAS V89 pp2679-2683 (1992)
Bredenbeek, et al. Sem. Vir. V3 pp297-310 (1992)
Bredenbeek, et al. J. Vir. submitted,"Sindbis Virus Expression Vectors
Packaging of Replicons using Defective Helper RNAs.
Bear in mind that when studying antigenicity, especially B-cell epitopes, the
most important characteristic to look for is an expression system which will
closely mimic the in vivo folding/post-translational modification of you Ag.
Thus, for quality, mammalian cells can't be beat. With these new systems, high
levels (rivalling bacterial expression levels) can be obtained.
Good luck.
Brett Lindenbach
Washington University - St. Louis
PS I am only sending this to you directly. Feel free to forward this to the
net if you find it interesting.
