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Growing macaque T cells

Michael Robertson michaelr at u.washington.edu
Thu Feb 10 17:40:14 EST 1994

We are trying to generate CTL from SIV and HIV-2 infected macaques.  To 
support macaque T cells in culture, we and others found that recombinant 
human IL-2 did not work very well but that a conditioned medium (PHA sup 
from human PBMC--a commercial product we got from Schiapparelli 
Biosystems, Inc) was very good for growing cells.  Unfortunately the 
cells very quickly seem to develop LAK-like killing.  Does anyone have 
any experience with this?  Specific questions:

1)  Do macaque NK or LAK cells kill K562 (which is how we measure this)
2)  Are there better defined cytokines for growing macaque T cells
3)  Would it help to decrease the percentage of conditioned medium--we 
use it at 10%

Any thoughts would be appreciated

UW Retrovirus Lab
Seattle, WA

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