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titering in suspense

unknown at dl.ac.uk unknown at dl.ac.uk
Mon Jan 17 02:44:00 EST 1994

Hello, looking for any suggestions. How would one go about determining the
titer of an animal virus in mammalian cells grown in suspension? These cells
(lymphoblastoid lines) do not want to adhere to most things, and I am concerned
with being as quantitative as possible. I am not interested in looking for
replication (ie plaquing units), but would like to know how many cells express
these viral products (I have antibodies). I have considered immunoflourescent
microscopy but since these cells do not like to sit down, I do not see the
feasiblity of this approach. I am also looking into flow cytometry, but am
concerned with sensitivity. Also, several of the proteins I am interested in
are intracellular. Please post any response or mail me directly. I am...
much obliged!

Brett Lindenbach
Washington University - St. Louis
brett at borcim.wustl.edu

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