Hi,
I've recently become the proud owner of the CLONTECH BacPAK expression
system ;). The protocol accompanying the kit suggests using Lipofectin(tm)
to transfect the Sf21 cells with recombinant virus. I'm interested to hear
any thoughts or opinions from baculoviral-minded individuals on other
methods of transfecting Sf9/Sf21 cells, particularly electroporation.
Cheers,
Garry.
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Garry Myers Menzies School of Health Research
Molecular Genetics Unit garry at menzies.su.edu.au
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