In article <garry.25.00122E06 at menzies.su.edu.au>, garry at menzies.su.edu.au
(Garry Myers) wrote:
> Hi,
>> I've recently become the proud owner of the CLONTECH BacPAK expression
> system ;). The protocol accompanying the kit suggests using Lipofectin(tm)
> to transfect the Sf21 cells with recombinant virus. I'm interested to hear
> any thoughts or opinions from baculoviral-minded individuals on other
> methods of transfecting Sf9/Sf21 cells, particularly electroporation.
>> Cheers,
>> Garry.
I have never tried electroporation and I don't even know if Sf cells can be
transfected that way. Is there a DNA size limitation for electroporation?
The baculovirus DNA is about 130kb. Lipofectin works well. CaPO4 is less
efficient but it also works. Good luck.
--
Steve "Some day I will get the hell out of Wisconsin" Rodems
"Then I am here for the Lee family renioun ... shur-wajo-shur"
