IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

Transfection of Sf9/Sf21 Cells

Steve Rodems smrodems at students.wisc.edu
Sun Jun 5 22:14:06 EST 1994

In article <garry.25.00122E06 at menzies.su.edu.au>, garry at menzies.su.edu.au
(Garry Myers) wrote:

> Hi,
> I've recently become the proud owner of the CLONTECH BacPAK expression
> system ;).  The protocol accompanying the kit suggests using Lipofectin(tm)
> to transfect the Sf21 cells with recombinant virus.  I'm interested to hear 
> any thoughts or opinions from baculoviral-minded individuals on other 
> methods of transfecting Sf9/Sf21 cells, particularly electroporation.
> Cheers,
> Garry.

I have never tried electroporation and I don't even know if Sf cells can be
transfected that way.  Is there a DNA size limitation for electroporation? 
The baculovirus DNA is about 130kb.  Lipofectin works well.  CaPO4 is less
efficient but it also works.  Good luck.
Steve "Some day I will get the hell out of Wisconsin" Rodems

"Then I am here for the Lee family renioun ... shur-wajo-shur"

More information about the Virology mailing list

Send comments to us at archive@iubioarchive.bio.net