In article <9406061709.AA12133 at unixsrv1.lsumc.edu>, mfreis at lsumc.edu
(Marion Freistadt) wrote:
>> I am looking for plasmids encoding expressed forms of either hygromycin or
> histidinol resistance for dominant selectable markers in eukaryotic
> transfection. Can anyone tell me where such plasmids are available?? I
> have already tried the major biotech supply companies--invitrogen,
> clonetech, promega, strategene, pharmacia, gibco. Thank you.
>British Biotech do a range of hygromycin and histidinol selection vectors.
British Biotechnology Ltd
Phone +44 865 781045
Fax +44 865 717598
Also, there's a vector by the name of pSV2-hyg originally by P. Berg for
hygromycin resistance. The EBV oriP vectors developed by B. Sugden
(Madison, U of Wisconsin) used hygromycin as well. Hyg-selection vectors
are so common I think you should be able to get them off colleagues in most
major med schools.
David Huen
