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Two Questions

CAS bioldv at pop.uh.edu
Tue Jun 7 19:04:00 EST 1994

Chris Seid
Dept.of Biology
Univ. of Houston
bioldv at pop.uh.edu

2. Mlu I ends are compatible with BssHII ends which flank
both ends of the multiple cloning site present in Stratagene's
Bluescript vector. You could essentially cut out the MCS from
Bluescript with BssHII and ligate in your fragment. For sequence
analysis you would have to use M13-20 primer. 

Unfortunately the T3 and T7 primers would be eliminated which
would prevent you from generating sense and antisense transcripts
from your sequence. If sequencing is your main motive for cloning,
this would be the way to go.

Good Luck.

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