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Walter Ogston ogston at HOBBES.KZOO.EDU
Wed Jun 29 10:11:28 EST 1994

Robert Coelen asks:
> One of my students cam across a few papers using freon (as in the subject 
> line) to extract virus containing clarified supernatant. She asked the
> very reasonable question waht is it used for ? I haven't used it myself,
> so I am asking you !
My understanding, based on first principles not on any expert source, is
that freon serves as a nonpolar extraction solvent.  Perhaps it extracts
cell membrane lipid without disrupting the virus.  Are these enveloped
or non-enveloped viruses?  
Walter Ogston				ogston at hobbes.kzoo.edu
Department of Biology			Phone: (616)337-7010
Kalamazoo College			Fax:   (616)337-7251
Kalamazoo, MI 49006-3295

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