Robert Coelen asks:
>> One of my students cam across a few papers using freon (as in the subject
> line) to extract virus containing clarified supernatant. She asked the
> very reasonable question waht is it used for ? I haven't used it myself,
> so I am asking you !
>My understanding, based on first principles not on any expert source, is
that freon serves as a nonpolar extraction solvent. Perhaps it extracts
cell membrane lipid without disrupting the virus. Are these enveloped
or non-enveloped viruses?
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Walter Ogston ogston at hobbes.kzoo.edu
Department of Biology Phone: (616)337-7010
Kalamazoo College Fax: (616)337-7251
Kalamazoo, MI 49006-3295
