I am posting this for a friend:
I am interested in putting the green fluorescent protein from the
bioluminescent jellyfish Aequorea victoria into adenovirus for
infection of mammalian cells. I have heard that the stability of this
protein in mammalian cells has been somewhat of a problem. Has
anyone had any experience with this and/or constructed more
stable expression vectors for this protein in mammalian cells?
Thanks in advance for any responses.