In article <2rrmho$pcd at news.u.washington.edu>, frv at u.washington.edu (Franklin Vincenzi) writes:
> Dr. J.P. Clewley <jclewley at crc.ac.uk> wrote:
>> Some helpful advice, and then
>> >What does anyone else think?
>> I think I'm glad I asked what the lot of you think about this! Thanks
> for the crash course.
>> Regards, Franklin
One thing I forgot to mention is that its more difficult to engineer
a negative strand virus than a positive strand one. If its +ve, you
can make a cDNA clone & transfect it in cells & recover progeny virus.
Works for alphaviruses (eg. Sindbis) picornaviruses (e.g. polio),
retroviruses (e.g. HIV). Recovering -ve strand virus from transfected
DNA has not been done reproducibly, as far as I know. Is there a
confirmed example I'm missing?