> From: lehn at facstaff.wisc.edu> In article <MAILQUEUE-101.941005094123.448 at molbiol.uct.ac.za>,
> <ED at molbiol.uct.ac.za> writes:
...
> > > major plot device is an unusual form of airborne virus. I
would
> >
> > The only kind of unusual form of airborne virus would be one
that is
> > totally unrealistic, ....
>> Not so fast here. Suppose for the sake of argument, that one
> were to insert the HIV genome into another virus......
>> Lets see now, if HIV were cloned into vacinnia and the virus were
> grown up in large quantities and spayed in a fine aerosol over
> a large metropolitin area. Eeeeeh! ...
Yeah, well, I was talking about NATURAL viruses here...and watch
out, if the Rev. James reads this, he will report you to his cousin
the law enforcement officer...! But that really WOULD be a nasty,
wouldn't it? Under the control of a vaccinia late promoter,
engineered so it initiated and terminated appropriately - and would
presumably express appropriately from vaccinia. BUT what about
splicing? Wouldn't get appropriate splices, wuld you, not being in
the nucleus and all? Would presumably make gag and maybe pol OK,
but not env...unless the pol could work on non-encapsidated RNA, and
you got appropriate genomic insertion, THEN the normal retrovirus
expression. Sounds like a "shoot when receive grant proposal" type
of project to me!
_________________________________________________________________
| Ed Rybicki, PhD | Well, I tip my hat |
| (ed at micro.uct.ac.za) | To the new constitution |
| Dept Microbiology | Take a bow for the new revolution... |
| University of Cape Town | Then I get on my knees and pray |
| Private Bag, Rondebosch | We don't get get fooled again... |
| 7700, South Africa | |
| fax: xx27-21-650 4023 | - Pete Townshend, 1972 |
| tel: xx27-21-650 3265 | (Won't get fooled again) |
------------------------------------------------------------------
