In article <38krbk$gac at usenet.INS.CWRU.Edu> zxy5 at po.CWRU.Edu (Zafer Yildirim) writes:
>>I know that , for example , vaccinia viruses have been used in
>transfer of foreign genes into cells. The gene is incorporated
>into the viral genome by homologous recombination from a plasmid
>if I am not wrong. Has anybody tried this or any other method to
>incorporate genes into the herpes virus genome ? Which part of
>the viral genome is targeted in general ? How stable would it
There has been a great deal of work on using herpes viruses (primarily
herpes simplex virus) as expression vectors. A number of of methods and
genomic regions are used, depending on what you want to do. In my former
lab, I made a number of plasmids for introduction of foreign genes into
HSV, and the procedures were quite straightforward and reasonably
efficient. Other labs have also made many plasmids (I like mine best,
and of course I'm completely unbiased). E mail me if you would like more
information.
Ian
--
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-4328 Fax (617)-632-2627
